In conjunction with appropriate staining, laser capture microdissection (LCM) enables very specific targeting of cell types. Our in-house pathologist leads our LCM team that has extensive experience with tissue sectioning, staining, and capturing target cells. We use a Zeiss PALM system to image, cut and catapult target cells. Before and after images of the tissue section are stored and used for QC pass/fail determination. Gene Logic’s RNA isolation team use optimized and validated protocols and prepare RNA for successful gene expression analysis.
Process: After receiving an order, our project management team will contact you and confirm details. Expectations will be set and the project manager will provide you with frequent status updates.
QC: Gene Logic has established QC tests for nucleic acid isolation and LCM handling. From quantity to quality metrics to sample images, this information is delivered to clients. We have several QC check-points and share results with client at each point
Reports: At each QC step, results are shared with client. Final report includes all platform specific experimental and QC data in appropriate format. Results are delivered by secure data transmission, DVD, CD, removable hard drive or any other mechanism mutually agreed upon with client.