Arabidopsis thaliana is a small flowering plant that is widely used as a model organism in plant biology. Although it is a non-commercial member of the mustard family, it is favored among basic scientists as it develops, reproduces, and responds to stress and disease in much the same way as many crop plants.
Because of its small genome size (125 Mb total; completely sequenced in the year 2000), a rapid life cycle, efficient transformation methods, and a large number of mutant lines, it offers important advantages for basic research in genetics and molecular biology.
Experimental Design
4 - 5 week old
A. thaliana plants were sprayed and watered with 1 mM salicylic acid and harvested after 24 hours in liquid nitrogen. Extraction and precipitation of RNA was performed using TriZol (GibcoBRL, Invitrogen, Karlsruhe, Germany) according to the manufacturer's instructions. RNA was labeled using a target amplification method. We designed an
Arabidopsis Oligo Test Set containing 96 oligonucleotides representing general plant stress genes, PR marker genes, and housekeeping genes of
A. thaliana, as well as several rat genes as negative controls. In order to test the performance of
Arabidopsis Oligo Test Set, labeled cRNA (target amplification method) was hybridized to the array.
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Unique Advantages
Gene specific HPSF® oligonucleotide probes High reproducibility Extensive quality controls - Probes by MALDI-TOF MS - Arrays by SYBR® Green I staining and random hybridization |
OciChip™ array Concept
