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Sequencing

Next-Gen Data Analysis
Sequencing Services – Enabling Cost-Effective Quality Sequencing

Ocimum offers high throughput sequencing services and solutions to all core and research laboratories in academic, government, and medical institutions; as well as biotech and pharmaceutical laboratories.


OVERVIEW

Using the gold standard 96-capillary 3730xl DNA Analyzer, We provide various DNA analysis applications such as microsatellites, SNP analysis, BAC fingerprinting, mutation detection, resequencing, LOH (loss of heterozygosity) and traditional DNA sequencing for animal, plant, and microbial genomes.

With our team of trained bioinformaticians, sequencing experts, and statisticians we can help you to achieve optimized assembly, accurate SNP analysis and better base calling. Our expert team also offers assistance for your genome sequence annotation and analysis, mutational analysis, and confidential sequencing data management.

Samples:

You can send us plasmid DNA or PCR products, BACs, cosmids, PACs, small genomes, or purified viral particles, soil, water and tissue sample.

Report and Data:

Final report contains raw data, assembled data, chromatograms and data analysis report (optional).

Support and Confidentiality:
p>Being GLP compliant and ISO certified company, we are committed to provide you with the best 24/5 technical support and to maintain your data confidentiality.

SERVICES OFFERED

Shot Gun Sequencing (BACs/Cosmids)

Bacterial artificial chromosomes (BAC)/cosmids are highly stable, specific markers and preferred constructs for high-throughput genomic sequencing of organisms with long genomes. BAC end terminals provide highly specific signature/markers, but due to the large size of BAC DNA (>100 Kbp), the sequencing process becomes difficult and is more inclined to secondary structure, inaccurate base calling and bad read length.

Ocimum’s BAC/Cosmid optimized fingerprinting services provide:

  • Secondary structure and bad read free data generation
  • Cost-effective optimized data generation
  • Bioinformatics assembly and sequence annotation support

Primer Walking

DNA fragments longer than 2000 bases have always been difficult to convert or sequence in a read. Sequencing methods such as Primer Walking are pivotal for these long DNA fragments. Instead of long reads, this method provides short consecutive stretch.

In Primer Walking, the primers first get annealed at the start of DNA fragment and then get extended. Ultimately, this generates short stretch reads using chain termination method. The resulting short reads act as primer for generating the next read. In this fashion, primer walking generates sequence data in small reads.

Ocimum’s primer walking service provides:

  • Designing and synthesis of custom primers
  • Use of universal primers or your pre-designed primers
  • Quality controlled data generation
  • Assembly data analysis and management

Re-sequencing (SNP profiling)

When initial sequencing data is available for a given genome, it can then facilitate re sequencing experiments to identify single nucleotide polymorphisms, structural variations and mutations among sequences. Re sequencing project often require highly parallel systems to detect variations.

The re-sequencing services provided by Ocimum facilitates:

  • High throughput architecture
  • Multiple coverage across reads
  • Accurate identification of SNPs, Indels and copy number variations
  • Less time and resource consumption
  • In house design and synthesis of re-sequencing oligos
  • Quality controlled data generation
  • Comprehensive data analysis and management services

High throughput sequencing

High-throughput DNA sequencing technology allows much faster and more cost-effective sequencing than traditional Sanger sequencing. A standardized protocol can be used for parallel sequencing of a number of samples.

The high throughput sequencing service at Ocimum facilitates:

  • Genomic sequencing
  • cDNA sequencing
  • Amplicon sequencing
  • Design and synthesis of sequencing primers
  • Base calling and verification
  • Vector trimming and read clean up
  • Read assembly and correction
  • Comprehensive data analysis and management services

Bacterial Identification

All bacteria comprise 16s ribosomal gene, which contains conserved and hypervariable nucleic acid sequences. Studies have shown that a 16S rRNA gene sequence similarity in the range of 0.5 to 1% is required for the identification of a bacterial species. These 16srRNA will also help in constructing the phylogenetic relationship and antibiotic susceptibility profile of a microorganism.

These sequences can be utilized for identification of bacteria using DNA sequencing method. DNA sequencing is accurate and fast, while traditional phenotypic methods have low accuracy and reproducibility.

Ocimum provides you:

  • Genomic DNA isolated from the pure culture pellet
  • Genomic sequencing from ready-made culture plates
  • Design and synthesis of sequencing primers
  • Phylogenetic analysis using bioinformatics support

Get in touch with us:
telephone+91-40-6698-6700
fax+91-40-6662-7205
emailinfo@ocimumbio.com
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